Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Martinez LM[original query] |
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Evaluation of Two Real-Time, TaqMan Reverse Transcription-PCR Assays for Detection of Rabies Virus in Circulating Variants from Argentina: Influence of Sequence Variation.
Caraballo DA , Lombardo MA , Becker P , Sabio MS , Lema C , Martínez LM , Beltrán FJ , Li Y , Cisterna DM . Viruses 2020 13 (1) In rabies diagnosis, it is essential to count on a rapid test to give a quick response. The combined sensitivity and robustness of the TaqMan RT-PCR assays (qRT-PCR) have made these methods a valuable alternative for rabies virus (RABV) detection. We conducted a study to compare the applicability of two widely used qRT-PCR assays targeting the nucleoprotein gene (LysGT1 assay) and leader sequences (LN34 qRT-PCR assay) of RABV genomes, in all variants circulating in Argentina. A total of 44 samples obtained from bats, dogs, cattle, and horses, that were previously tested for rabies by FAT and conventional RT-PCR, were used in the study. All variants were successfully detected by the pan-lyssavirus LN34 qRT-PCR assay. The LysGT1 assay failed to detect three bat-related variants. We further sequenced the region targeted by LysGT1 and demonstrated that the presence of three or more mismatches with respect to the primers and probe sequences precludes viral detection. We conclude that the LysGT1 assay is prone to yield variant-dependent false-negative test results, and in consequence, the LN34 assay would ensure more effective detection of RABV in Argentina. |
Applying lessons learned from CDC's health literacy initiatives
Martinez LM . Stud Health Technol Inform 2020 269 341-347 This report describes health literacy initiatives at the Centers for Disease Control and Prevention (CDC) and the lessons learned from more than 20 years of commitment to health literacy. Efforts described include CDC's training and tools, the Science Ambassador Fellowship program, and an action plan and report card. The report concludes with recommendations on how to meet the needs of communities and systems. |
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